Confocal Laser Scanning Biological Microscope FV300 : Specifications (2)
Specifications
| Item | Specifications | |
|---|---|---|
| Laser light source | Visible light laser source | Select from the following laser, to mounted on laser combiner Multi-line Ar laser (458nm, 488nm, 515nm, Total 40mW), Ar laser (488nm,10mW), HeNe (G) laser (543nm,1mW), HeNe (R) laser (633nm,10mW), LD440 (440nm, 5.3mW) |
| LD405 laser | direct fiber connection to scan unit | |
| Laser combiner | Each laser light path is equipped with a continuously variable neutral
density filter or AOTF All laser lines are combined to apsis along the same fiber optic |
|
| Scanning unit | Scanning method | Galvanometer mirror scanners (both X and Y) |
| Field number | 20 (10 with use of LD405 laser) | |
| Pinhole | 5-position pinhole turret | |
| Image memory and scanning speed | Standard scanning mode: 256 x 256 (0.45s) - 2048 x 2048 (10.835s) Bi-directional high-speed scanning mode: 512 x 512 (0.25s) (Simultaneous scanning of up to 2 channels) |
|
| Image channel | Selectable from 2-channel (fluorescence) or 2-channel (fluorescence)
+ 1-channel (transmitted light) 3-channel (fluorescence) using virtual channel |
|
| Selection of filters according to staining | Manual selection | |
| Scanning modes | XY, XYZ, XYT, XYZT, XZ, XT, XZT, point, Line-t, free line-t, line-z, free line-z, Clip, ZoomIn | |
| Image depth resolution | 12-bit (=4096 grey levels) | |
| Zoom | 1X-10X | |
| Z-drive | Step motor/Minimum step 10nm (BX61, BX61WI and IX81 combination), 25nm (other microscope combination) | |
| Microscopes | Upright | BX51, BX61, BX51WI, BX61WI |
| Inverted (special laser safe frame) | IX81FVSF, IX71FVSF (side port) | |
| External transmitted light unit | Transmitted light illumination unit | External halogen light source connected to microscope via fiber cable |
| Transmitted light detector | External detector unit with built-in photomultiplier Connected to microscope frame via fiber cable | |
| Fluorescence illumination unit | Connect to external mercury light source and microscope via fiber
cable Standard equipment of FV300-BX51, FV-300-BX61, FV300-BX51WI, FV300-BX61WI |
|
| PC with system control boards | PC-AT compatible machine/OS:
Windows XP (English version)/ 1GB memory
(can be expanded to a maximum of 4GB) CPU: Pentium 4 over 3.2GHz, Special I/F board/image capture: PCI bus Graphic board: Radeon X1300Pro/256MB Hard disk: 120GB, Optical Drive: DVD-ROM Monitor: Two 19” LCD monitors are recommended, each able to display 1280x1024 images in full color (16.77 million colors) LAN: On board |
|
| Fluoview application software | Image acquisition | Scanning condition setting: image size, scanning speed, zoom, panning
etc. Real-time image calculation: Kalman filtering, peak integration, |
| Hardware control | Laser, scanning unit, microscope | |
| Image display | Each image display: Single-channel side-by- side, merge, cropping,
tiling, series (Z/T) pass and continuous LUT: Individual color setting, pseudo-color, Overlay: Lines, text, scale bar, etc |
|
| Image processing | Individual filter: Average, Low-pass, High-pass, Sobel, Median, Prewitt,
2D Laplacian, edge enhancement etc. Calculations: Inter-image, mathematical and logical, DIC back ground leveling |
|
| Image analysis | Overview of fluorescence intensity within an area, histogram, perimeter measurement for user-assigned area, time-lapse measurement , etc. | |
| 3D visualization | 3D animation, left / right stereo pairs, red / green stereoscopic images and cross section | |
| Others | Graphic-based help, PAPP (Programmable Acquisition Protocol Processor), time course software (optional), trigger IN/OUT function (optional), Multi point software (optional) | |
| Power consumption | Microscope (115V 6A/230V 3A), scanning unit+PSU (115V 3.5A/230V 2A), computer & monitor (115V 4.5A/230V 10A), Ar laser (115V 10A/230V 5A), Mult-linei Ar laser (115V 10A/230V 5A), Kr laser (230V 20A), HeNe laser each (115V 0.4A/230V 0.2A), LD laser (405nm, 440nm: 100V 0.9A/230V 0.5A) | |
