Confocal Laser Scanning Biological Microscope FV10i-LIV/FV10i-DOC : Applications
Observation of antibody dependent cellular cytotoxicity (ADCC)
[New Application]
(PDF:1.54MB)
- RPMI 4788 cells (human colon cancer cell line) were treated with an antibody drug, cetuximab, and co-cultured with
natural killer (NK) cells
- ADCC was observed using the FV10i after addition of NK cells
- Cetuximab: Alexa Fluor 647 (red)
- NK cells: ZsGreen (green)
- Detection of dead cells: DAPI (blue)
Images courtesy of:
Clinical Chemotherapy Department
The Cancer Chemotherapy Center of the Japanese
Foundation for Cancer Research
Dr Yuji Mishima, Dr Kiyohiko Hatake
Image acquisition by Dr. Takashi Abe (Olympus Corporation)
Hela cell
Time Interval
Every 20 minutes during a night
Green: GFP
Magenta: Mito Tracker Red
NG108-15 cell
Green: Actin (Alexa Fluor488-Phaloidin)
Magenta: Mitochondria (Mito Tracker Red)
Hela cell
Cyan: Core (DAPI)
Green: Microtubule (Alexa Fluor488)
Magenta: Mitochondoria (Mito Tracker Red)
NG108-15 cell
Green: Actin (Alexa Fluor488-Phaloidin)
Magenta: Mitochondoria (Mito Tracker Red)
A549 cell
Cyan: Core (DAPI)
Green: Microtubule (Alexa Fluor488)
Magenta: Mitochondoria (Mito Tracker Red)
P12 cell
Green: Core (YOYO-1)
Magenta: Actin (Rhodamine-Phaloidin)
