Multiphoton Laser Scanning Microscope FV1000MPE
By closely adhering to optics principles and designing a microscope that is both compact and easy to use, Olympus developed the new FV1000MPE so that even more researchers can use the microscope to perform in-depth observation.
Special Features
- Characteristic of FV1000MPE
- Dedicated objective
- Application to the laser light stimulation
- lineups
What is multiphoton laser scanning microscope
By using femtsecond laser multiphoton is enabled, and the observation of the deep is possible than before.
The following specimen is the cerebral cortex of M-line, a strain of transgenic mouse (GFP), was exposed and from the surface in vivo imaging was performed with excitation of 488 nm for single photon and with excitation of 920 nm for multiple photons. With single photon, depths to only 400 µm can be observed, but with multiple photons depths to about 750 µm can be observed.
Specimens provided by:
Kimihiko Kameyama, Tomoyo Ochiishi, Kazuyuki Kiyosue, Tatsuhiko Ebihara
Molecular Neurobiology Group, Neuroscience Research Institute,
National Institute of Advanced Industrial Science and Technology, Japan
