Biological Microscopes

SCALEVIEW Immersion 25x Objective for Multiphoton XLPLN25×SVMP

Specially designed to deliver optimum performance with SCALEVIEW immersion, this dedicated multiphoton objective with an ultra-long working distance enables high-precision imaging of transparent-rendered biological specimens to a depth of 4 mm.

Optical Clearing Agent SCALEVIEW-A2

SCALEVIEW-A2 revolutionizes the imaging of formalin-fixed specimens. Simply render mammalian brain tissue transparent through immersion in SCALEVIEW-A2 solution. Because SCALEVIEW-A2 eliminates light scattering, it doesn't decrease the intensity of signals emitted by fluorescent proteins in the tissue and so enables structures labeled with fluorescent proteins to be imaged in detail from the surface to significant depth, without the need for mechanical sectioning.
SCALEVIEW-A2 contains the fundamental components of ScaleA2 developed by the RIKEN Institute. It is adjusted to achieve the optimal performance with the XLPLN25×SVMP objective.

Image data courtesy of:
Hiroshi Hama, Hiroshi Kurokawa, Atsushi Miyawaki
Laboratory for Cell Function Dynamics
RIKEN Brain Science Institute

Reference material:
Hiroshi Hama, Hiroshi Kurokawa, Hiroyuki Kawano, Ryoko Ando,
Tomomi Shimogori, Hisayori Noda, Kiyoko Fukami,
Asako Sakaue-Sawano & Atsushi Miyawaki
Scale: a chemical approach for fluorescence imaging and reconstruction of
transparent mouse brain
Nature Neuroscience, advance online publication, 30 August 2011
(doi:10.1038/nn.2928)