Biological Microscopes

Focus drift compensation function for time-lapse experiments.

Motorized inverted research microscope with focus drift compensation/IX81-ZDC
This landmark microscope model makes it easy to reproduce any preset focus position. 785nm weak laser light is introduced through the additional optical path between the tube lens and an objective to measure the distance between the objective and the reflection plane, which is normally the boundary of the reflective index difference such as the boundary between cover glass and cell. Therefore this system never cause unnecessary photobleaching of the specimen.

CO2 incubators / MIU-IBC-I, MIU-IBC-IF

Highly precise incubator control keeps the environment inside a laboratory dish completely stable, at just below 37°C temperature, 90% moisture and 5% CO2 concentration (when using a CO2 5% concentration bomb); in this way, live cell activity can be maintained for about 2 days. A special designed structure is employed to minimize the focus drift during temperature control. This is the ideal solution for time-lapse experiments under both a confocal laser scanning microscope and a wide field observation. The opening hole located on the top heater is available for the cell injection.
* Built-in stage warming plate
* Objective heater
* 5% CO2 supply tube with ø4 outer diameter, ø2 inner diameter and 400mm length.
* Not available in some areas

MIU-IBC-I
Basic configuration which allows control of heaters for top, bath, stage and objective.

MIU-IBC-IF
This configuration includes a built-in flowmeter for 5% CO2 and 95% air. Use the 5% CO2 and 95% air bombs.

Incubators

This box type incubator keeps the microscope temperature stable with enclosing many components inside the box. Please see your local supplier for more detail.

Thermoplate / MATS series

This thermoplate maintains the cell specimen container temperature at 37°C.
* Tokai Hit Company products

Frame plate adapter / IX2-FP

This is used to fix the microscope body to the antivibration stand.
*Screws (available separately) are required for fixing.

Nosepiece stage/IX2-NPS

This simple mechanical stage is designed for long time observations to minimize the distance change between the specimen and the objective in other words 'focus drift'. It works by minimizing the effect of temperature change and prevents blur during long observations. Attach one objective in use.

Comparison of normal observation images

Change of focus when environmental temperature (25°C ) changes by ±5°C.
* When the microscope is used without an incubator.

Environmental temperature	20°C	25°C	30°C
Normal observation images
With IX2-NPS

Comparison of fluorescence images

Time-lapse observation images.
*When the microscope is used without an incubator.

Elapsed time(min)	0	30	60	120
Normal observation images
With IX2-NPS