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Research Inverted System Microscope IX71/IX81 : Features (6)

Special equipment for relief contrast and phase contrast.

Relief contrast equipment

The Olympus Relief Contrast system provides a high contrast, 3-D image similar to DIC for specimens mounted in plastic vessels. Relief contrast is designed for use in cellular fertilization and making the nuclear envelope easier to see and penetrate.

Relief contrast equipment
* Unifies the shadow directions of each objective, improving operability at all magnifications.
* Maintains a long working distance (45mm) for the condenser (IX2-MLWCD) so as not to hinder operation of the manipulator.

Two types of objectives for relief contrast are selectable: costefficient Achromat models, or PlanSemiApochromat objectives with high resolution and excellent focusing right up to the image perimeters. Condenser (IX2-MLWCD) also supports DIC and phase contrast observations for maximum flexibility.

Objectives for Relief Contrast observation

  N.A. W.D.
Achromat for
Relief Contrast
CPLN 10XRC *1 0.25 9.7mm
LCACHN 20XRC *1 0.40 2.8mm
LCACHN 40XRC *1 0.55 1.9mm
Plan Fluorite for
Relief Contrast
CPLFLN 10XRC *1 0.30 9mm
LUCPLFLN 20XRC *2 0.45 6.6 — 7.8mm
LUCPLFLN 40XRC *2 0.60 3.0 — 4.2mm

*1 Objective with compensation for 1mm plastic dish plus 0.5mm thick thermoplate *2 Objective with compensation ring for 0~2mm thick cover glass.

DIC optical elements for IX2-MLWCD and applicable objectives

Optical elements Objectives
IX2-MDIC20 UPLSAPO20X, UPLFLN20X, LUCPLFLN20X
IX2-MDIC40 UPLSAPO40X2, UPLFLN40X, UPLFLN40XO* , LUCPLFLN40X

* Use with shift DIC slider (U-DICTS).

Phase contrast optical elements for IX2-MLWCD and applicable objectives

Optical elements for
IX2-MLWCD
Applicable objectives
IX2-MPHL UPLFLN4XPH
IX2-MPHC CPLFLN10XPH, CPLN10XPH, LCACHN20XPH
IX2-MPH1 LUCPLFLN20XPH
IX2-MPH2 LUCPLFLN40XPH, LCACHN40XPH, LUCPLFLN60XPH

* Use with shift DIC slider (U-DICTS).

relief contrast equipment

The IX2-MLWCD comes equipped with optical component RC1 (for 10X objective), RC2 (for 20X objective), RC3 (for 40X objective) and a polarizer to adjust the contrast.


Phase contrast equipment

Phase contrast equipment

Ultra long working distance condenser / IX-ULWCD
This universal condenser for phase contrast and brightfield observations offers excellent workability due to its long working distance (73mm) and compatibility with large containers: it can be used in combination with 4X -40X phase contrast objectives. Phase contrast observation is also possible with the IX2-LWUCD condenser, whose working distance is 27mm.

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