Biological Microscopes
- Scanning Units
- Scanning
- Sequential scanning
- Software
- 3D Imaging
- Time Course
- Fluorescence Dyes
- Objectives
Time Course
Using different scanning modes to chart time-lapse changes efficiently
High-speed (4 frames/sec) image acquisition
The unit's high-speed image capturing mode provides 4 confocal frames (512x512 pixels) per second. This bi-directional fast scanning mode can be used to record rapid changes such as calcium ion density.
Versatile line scanning modes has many uses
The wide range of line scanning modes (linear/oblique/free-line) enables flexible analysis of rapid time-lapse changes in the specimen.
High precision analysis of time-lapse changes
The wide dynamic range of 12-bit images with 4,096 gray levels covers subtile as well as dramatic changes in fluorescence. By using rectangles, circles or free-line areas, the operator can designate several regions of interest (ROI), observe and measure them simultaneously during (on-line plot) or following time lapse observations through the display of intensity versus time graphs.
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Calcium wave in Xenopus oocyte, Calcium Green staining,
fluorescence pseudo-colored fluorescence image after injection of inositol
3-trisphospate Japan Science and Technology Corporation, Exploratory Research for Advanced Technology, Mikoshiba cell control project, Prof. Aya Muto |
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Calcium wave in isolated cardiac myocyte Dr. Sandor Gyorke Texas Technical University |
Immersion-type LUMPLFL objectives
The 40X water immersion objective in this series has a 3.3mm working distance and an extremely fine tip which is suitable for micromanipulation using a fixed stage upright microscope. It has a large N.A. (0.8) and is also ideal for confocal observations. When using the BX51WI or BX61WI (fixed stage upright microscopes) with water immersion objectives confocal imaging can be used to monitor time lapse fluorescence changes in thick specimens such as brain slices.
Real-time kinetic measurement
Time course software permits the real-time display of intensity graphs during the acquisition of fluorescence images over time. Event markers may also be inserted during the experiment to correlate changes in experimental conditions.
Ratio imaging for analysis of 2-wavelength fluorescence images
Using the view processor function, time course software allows a series of images obtained for two different wavelengths to be displayed as a pseudo-colored ratio image. Extensive quantitative analysis of defined regions can be displayed using embedded graphing capabilities.
Input/output of external trigger signal
External devices such as those used in patch clamp experiments can be synchronized with image acquisition using the input and output of trigger signals.
