Confocal Laser Scanning Biological Microscope FV10i-LIV/FV10i-DOC : Specification
| FV10i-LIV | FV10i-DOC | ||
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| Laser light source | LD lasers: | 405nm(17.1mW), 473nm(11.9mW) ,559nm(15mW), 635nm(9.5mW) | |
| Modulation: | Continuously Variable by the LD direct modulation (0.1%-100%, 0.1% inclement) Line return period - laser OFF |
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| Scanning | Scanning method | 2 galvanometer scanning mirrors | |
| Scanning mode | Pixel size: 256 × 256 - 1024 × 1024 Scanning speed: 1.1 s / frame (for pixel size 512 × 512, High Speed scanning mode) Focusing scanning: High frame rate scan by Y- direction interlace scanning (×1, ×2, ×4) Dimension: XYT, XYZ, XYZT Rotation scanning: 0-359.9° in 0.1° increments |
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| Detection | Detector module | Fluorescence: 2 channels, Phase Contrast: 1 channel Variable barrier filter mechanism for fluorescence channel by diffraction grating and slit |
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| Detection method | Analog integration detection by Photomultiplier | ||
| Pinhole | Single motorized pinhone Pinhole diameter: ø50-800μm automatic setting (adjustable to ×1.0, ×1.5, ×2.0,×2.5,×3.0,×4.0, and ×5.0) |
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| Field number | 18 | ||
| Optical zoom | 10× objectives: 1× – 6× in 0.1× increments 60× objectives: 1× – 10× in 0.1× increments |
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| Automatic Exposure | Automatic Exposure Automatic setting of the laser intensity and photomultiplier sensitivity to fluorescence intensity | ||
| Focus | Z-drive | Motorized focus Minimum increment: 0.01μm |
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| Objectives | Exclusively designed 10× phase contrast objective / NA 0.4 (equivalent to UPLSAPO 10x) Exclusively designed 60× phase contrast water-immersion objective / NA 1.2 (equivalent to UPLSAPO 60× W) / with motorized correction collar Remote switching from software by electric revolver |
Exclusively designed 10× phase contrast objective / NA 0.4 (equivalent to UPLSAPO 10x) Exclusively designed 60× phase contrast oil-immersion objective / NA 1.35 (equivalent to UPLSAPO 60× O) Remote switching from software by electric revolver |
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| Automatic focus (AF) | Automatic detection of interface between specimen and cover glass by laser reflection light detection Automatic detection of cover glass thickness and automatic setting of motorized correction collar |
Automatic detection of interface between specimen and cover glass by laser reflection light detection |
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| Water supply | Automatic water supply and air cleaning mechanism for 60× Water-immersion objective |
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| Oil supply | Manual As supporting mechanism, automatic moving of XY stage to oil supply position when switching to 60x |
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| XY stage | XY driving method | Motorized XY stage module by stepping motor Minimum increment: 0.3μm |
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| Specimen holder | Only the dedicated specimen holder can be mounted For three glass bottom dishes with 35mm diameter For a glass slide, For one set of cover glass chamber (8 wells type) For Well slide (8 wells type), Culture pod(for a glass bottom dish with 35mm diameter) |
Only the dedicated specimen holder can be mounted For a glass bottom dish with 35mm diameter For a glass slide, For Well slide (8 wells type) |
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| Incubator | Room environment: | Temperature: 37+0.1°C,-0.5°C (can be switched off) Humidity: more than 90% CO2 concentration: 5% (recommended), 1 – joint fitting (ø2mm) for exterior CO2 adjustor |
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| Heating method | Non-contact heating by resistive heater mounted on frame section |
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| Control device | Controller | Dedicated controller PC/AT-compatible OS: Windows Vista Business, 32 bit (English version), CPU: Intel Core2Duo 3.0GHz RAM: 2GB × 2, HDD: 500GB × 2, Special PCI-Express I/F board built-in, Optical drive: DVD-Multi drive built-in |
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| LCD monitor | 24 inch LCD monitor × 1, WUXGA (1920×1200) | ||
| Main software feature |
Image acquisition mode | Map image, one shot, time-lapse (XYT), Z-stack (XYZ), Z-stack time-lapse (XYZT), multi area time-lapse (Multi Area XYT), multi area Z-stack time-lapse (Multi Area XYZT) |
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| Specimen setting | Automatic setting for fluorescence channel and laser according to Dye selected from Dye list | ||
| Map image acquisition | Automatic selection of map image of 3×3 – 9x9 fields according to 10× objective lens (The maximum area varies in accordance to the specimen holder used), and manual selection of map acquisition area | ||
| Multi area time-lapse | Automatic multi area time-lapse by motorized XY stage Setting for each registered point: Image size, scanning speed, cross talk reduction, pinhole diameter, rotation angle, galvano zoom, acquisition channel, laser power, PMT sensitivity, Z condition Maximum resister number: 10 items per one container Maximum interval time: one hour Maximum acquisition number of times: 3000 times per one point |
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| Image acquisition area | Area appointment: All area, clipping square area (minimum area: 96 × 96 pixels) | ||
| Image display | Display by channel, overlapping display, image in progress review | ||
| Cross talk reduction | Line sequential action (2 channel), or frame sequential action (3 channel and 4 channel) | ||
| Acquisition image file type | OLYMPUS image format (OIF) | ||
| Image file type available for viewing |
OLYMPUS image format (OIF, OIB), Multi-TIFF format (8/16 bit grey scale, index color, 24/32/48 bit color), JPEG, BMP, TIFF |
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| Image editing | LUT: pseudo color setting, contrast adjustment, Comment: inputting graphic, text, scale etc., image extraction, combination | ||
| 3D image construction | 3D display: AlphaBrend method, Maximum intensity projection method 3D animation display, free orientation of cross section display | ||
| Image processing | Various types of image filter: Median, Enhanced Edge, etc. Calculations: inter-image, arithmetic and logical operation |
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| Image analysis | Area and perimeter measurement, time-lapse measurement, colocalization analysis | ||
| Room environment |
Temperature | 18-28°C(fluctuation ±2°C) | |
| Humidity | 30-80% (non condensing) | ||
