Confocal Laser Scanning Biological Microscope FV10i : Specification
[W]=FV10i-W, [O]=FV10i-O
| Laser light source | Ultraviolet / Visible light LD lasers: | 405nm(18mW),473nm(12.5mW), 559nm(15mW),635nm(10mW) |
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| Modulation: | Continuously Variable by the LD direct modulation (0.1%-100%, 0.1% inclement) Line return period - laser OFF |
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| Scanning | Scanning method | 2 galvanometer scanning mirrors |
| Scanning mode | Pixel size: 256 × 256 - 1024 × 1024 Scanning speed: 1.1 s / frame (for pixel size 512 × 512, High Speed scanning mode) Focusing scanning: High frame rate scan by Y- direction interlace scanning (×1, ×2, ×4) Dimension: XYT, XYZ, XYZT Rotation scanning: 0-359.9° in 0.1° increments |
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| Detection | Detector module | Fluorescence: 2 channels, Phase Contrast: 1 channel Variable barrier filter mechanism for fluorescence channel by diffraction grating and slit |
| Detection method | Analog integration detection by Photomultiplier | |
| Pinhole | Single motorized pinhone Pinhole diameter: Ø50-800µm automatic setting (adjustable to ×1.0, ×1.5, ×2.0, and ×2.5) |
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| Field number | 18 | |
| Optical zoom | 10× objectives: 1× — 6× in 0.1× increments 60× objectives: 1× — 10× in 0.1× increments |
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| Automatic Exposure | Automatic setting of the laser intensity and photomultiplier sensitivity to fluorescence intensity. | |
| Focus | Z-drive | Motorized focus Minimum increment: 0.01µm |
| Objectives | Exclusively designed 10× phase contrast objective / NA 0.4 (equivalent to UPLSAPO 10x) Exclusively designed 60× phase contrast water-immersion objective / NA 1.2 (equivalent to UPLSAPO 60× W) / with motorized correction collar[W] Exclusively designed 60× phase contrast oil-immersion objective / NA 1.35 (equivalent to UPLSAPO 60× O) [O] |
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| Automatic focus (AF) | Automatic detection of interface between specimen and cover glass by laser reflection light detection Automatic detection of cover glass thickness and automatic setting of motorized correction collar[W] |
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| Water supply[W] | Automatic water supply and air cleaning mechanism for 60× Water-immersion objective | |
| Oil supply[O] | Manual As supporting mechanism, automatic moving of XY stage to oil supply position when switching to 60x |
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| XY stage | XY driving method | Motorized XY stage module by stepping motor Minimum increment: 0.3µm |
| Specimen holder | Only the dedicated specimen holder can be mounted For three glass bottom dishes with 35mm diameter[W], For one set of cover glass chamber (8 wells type)[W] Culture pod (for a glass bottom dish with 35mm diameter) [W], For a glass slide For a glass bottom dish with 35mm diameter[O] |
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| Incubator[W] | Room environment:[W] | Temperature: 37±1°C Humidity: more than 90% CO2 concentration: 5% (recommended), 1 - joint fitting (ø2mm) for exterior CO2 adjustor |
| Heating method[W] | Non-contact heating by resistive heater mounted on frame section | |
| Control device | Controller | Dedicated controller PC/AT-compatible OS: Windows Vista Business, 32 bit (English version), CPU: Intel Core2Duo 3.0GHz RAM: 2GB × 2, HDD: 320GB × 2, Special PCI-Express I/F board built-in, Media: DVD-Multi drive built-in |
| LCD monitor | 24 inch LCD monitor × 1, WUXGA (1920×1200) | |
| Main software feature | Image acquisition mode | Map image, one shot, time-lapse (XYT), Z-stack (XYZ), Z-stack time-lapse (XYZT), multi area time-lapse (Multi Area XYT), multi area Z-stack time-lapse (Multi Area XYZT) |
| Specimen setting | Automatic setting for fluorescence channel and laser according to Dye selected from Dye list | |
| Map image acquisition | Automatic selection of map image of 3×3 - 9x9 fields according to 10× objective lens, and manual selection of map acquisition area. | |
| Multi area time-lapse | Automatic multi area time-lapse by motorized XY stage Setting for each registered point: Image size, scanning speed, cross talk reduction, pinhole diameter, rotation angle, galvano zoom, acquisition channel, laser power, PMT sensitivity, Z condition Maximum resister number: 10 items per one container Maximum interval time: one hour Maximum acquisition number of times: 3000 times per one point |
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| Image acquisition area | Area appointment: All area, clipping square area (minimum area: 96 × 96 pixels) | |
| Image display | Display by channel, overlapping display, image in progress review | |
| Cross talk reduction | Line sequential action (2 channel), or frame sequential action (3 channel and 4 channel) | |
| Acquisition image file type | OLYMPUS image format (OIF) | |
| "Image file type available for viewing" |
OLYMPUS image format (OIF, OIB), Multi-TIFF format (8/16 bit grey scale, index color, 24/32/48 bit color), JPEG, BMP, TIFF |
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| Image editing | LUT: pseudo color setting, contrast adjustment, Comment: inputting graphic, text, scale etc., image extraction, combination | |
| 3D image construction | 3D display: AlphaBrend method, Maximum intensity projection method 3D animation display, free orientation of cross section display | |
| Image processing | Various types of image filter: Median, Enhanced Edge, etc. Calculations: inter-image, arithmetic and logical operation |
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| Image analysis | Area and perimeter measurement, time-lapse measurement, colocalization analysis | |
| Room environment | Temperature | 18-28°C |
| Humidity | 30-80% (non condensing) |
