Multiphoton Laser Scanning Microscope FV1000MPE : Specification
- Specifications
- Adapted lasers
- System layout example
| B system | S system | T system | M system | ||
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| Laser unit | IR pulsed laser with negative chirp for multiphoton excitation |
·Mode-locked Ti:sapphire laser [femtosecond laser (equipped with a group velocity dispersion correction/control device)],laser power unit, water-cooled circulating chiller ·MaiTai BB DeepSee-OL or MaiTai HP DeepSee-OL (Spectra-Physics products) MaiTai BB DeepSee-OL: 710 nm – 990 nm MaiTai HP DeepSee-OL: 690 nm – 1040 nm ·Chameleon Vision I-OL, Chameleon Vision II-OL (Coherent products) Chameleon Vision I-OL: 690 nm – 1040 nm Chameleon Vision II-OL: 680 nm – 1080 nm |
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| Visible light laser AOTF laser combiner |
LD laser: 405nm: 50mW, 440nm: 25mW, 473nm 15mW, 559nm 15mW, 635nm 20mW Multi Ar laser (458 nm, 488 nm, 515 nm, Total 30 mW), HeNe (G) laser (543 nm, 1 mW) Modulation: Continuously adjustable via an AOTF (0.1 – 100% in 0.1% increments) ·Operating mode: Allows laser turn-off during the retrace period REX: adjustment of laser power for each region, and selection of the laser and selection of the laser wavelength ·Visible light laser platform with implemented AOTF system, ultra-fast intensity with individual laser lines, additional shutter control, Connected to scanner via single-mode fiber ·Equipped with laser feedback mechanism to limit changes in laser light intensity over time |
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| Single laser for visible light | LD473 laser (15mW) Depending on the type of modulation: light intensity modulation, shutter control, corrected to the scanner via single-mode optical fiber |
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| Scanning unit | Scanning method | Light deflection via 2 galvanometer scanning mirrors | |||
| Scanning modes | ·Pixel size: 64 x 64 – 4096 x 4096 pixels
Scanning speed: (pixel time): 2 µs – 200 µs High-speed scanning mode: 16 frames/s (256 x 256) ·Dimensions: Time, Z, (wavelength) (or any combination thereof) ·Line scan: straight line (includes rotation), free line, point XY scan |
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| Zoom size | Observation position zoom with inclination width modification of galvanometer mirror: 1–50X (adjustable in 0.1X increments |
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| Confocal detector (The M scanner does not have a confocal detector) |
·Detector: 3 channels for fluorescence detection (photomultipliers), optional 4CH detector for expansion ·Dichromatic mirrors for excitation, dichromatic mirrors for multiphoton excitation, dichromatic mirrors for fluorescence, emission filter ·Infrared cut filter: using a high-performance filter ·A filter or spectral type of fluorescence detector can be selected Spectral type: Channels 1 and 2 provided with independent grating and slit Selectable wavelength range: 1 – 100 nm, wavelength resolution: 2 nm, wavelength switching speed: 100 nm/ms ·Pinhole: Single motorized pinhole, pinhole diameter: ø50 – 800µm (spectral type ø50 – 300µm), adjustable in 1 µm increments ·Field Number: 18 |
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| Optics with infrared laser for multiphoton imaging | ·Integrates a multiphoton near-infrared pulsed laser in the scanning unit (Laser safety measures implemented) ·Continuously variable output using AOM (0.1 – 100%, 0.1% increments) |
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| Component incorporating the laser for multiphoton imaging | Main scanner for observation | ASU scanner for laser light stimulation, Main scanner for observation: VIS laser | Incorporating
2 independent lasers for laser light stimulation/ observation |
M scanner for observation | |
| Detector for multiphoton imaging | Reflected light fluorescent detector | Photomultiplier (2 or 4 channels), Fluorescence wavelength can be selected with the dedicated filter cube (replaceable) |
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| Transmitted light fluorescent detector | Photomultiplier (2 channels), Fluorescence wavelength can be selected with the dedicated filter cube (replaceable) (not combinable with IX-SVL2) Exclusive equipment for the BX61WI upright microscope |
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| Transmitted DIC unit | ·Integrated transmitted light detector and transmitted illuminator, Motorized switching Connected to microscope via fiber cable (IR-DIC observation using an infrared laser is not possible) |
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| Z-drive | ·A motorized focus module inside the microscope is used ·Minimum increment: 0.01µm |
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| Microscope | Upright microscopes: BX61WI, BX61 Inverted microscope: IX81 | ||||
| System control | ·OS: WindowsXP Professional (English version), WindowsVista (English version) ·CPU: Core2Duo 2.33GHz ·Memory: 2.0GB or larger ·Hard disk: 320GB or larger ·Dedicated I/F board: built-in PC ·Graphics board: Open GL-compliant ·Recording media: Equipped with DVD dual drive |
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| Software | FV10-ASW Ver.2.0 or later | ||||
| Required installation environment | Room temperature: 25°C ± 1°C, humidity: 60% or less@25°C, dust level: Class 10000, requires continuous (24-hour) power supply |
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| Vibration isolation table for microscope and laser installation, size | 1500 mm x 1250 mm | 1500 mm x 1500 mm |
1700 mm x 1700 mm |
1500 mm x 1250mm | |
