Biological Microscopes

XLPLN25xWMP, dedicated objective with exceptional brightness and resolution for multiphoton imaging.

This water immersion objective with a high N.A. and widefield design has improved transmittance articularly of near-infrared wavelengths to optimize multiphoton fluorescence microscopy. this allowing formation of a focal spot even without reducing energy density even during in-depth observation. Its widefield design, which can detect scattered fluorescence in a specimen, allows extremely bright, high-resolution fluorescence microscopy.

Fluorescence detectors for high-sensitivity multiphoton imaging.

• *Reflected light fluorescence detector.
  This detector can capture all fluorescent signals without fluorescence passing through a confocal aperture, so this allows imaging at a high level of sensitivity that minimizes light decay.

• Fluorescence detectors for high-sensitivity multiphoton imaging.
  *Reflected light fluorescence detector.
  This detector can capture all fluorescent signals without fluorescence passing through a confocal aperture, so this allows imaging at a high level of sensitivity that minimizes light decay

What is Second Harmonic Generation (SHG)?  

Second Harmonic Generation imaging of neurons.

A: SHG image of neurons in dissociated culture from the mouse cerebral cortex. After FM4-64 was administered to neurons from outside the cells, the cells were irradiated with a femtosecond laser at 950 nm and the SHG signal at 475 nm was detected with the transmitted light detector.
B: An enlargement (5x) of the portion in the yellow box in image A. As is apparent, spines protruding from dendrites can be observed with SHG.
C: SHG and multiphoton images have been superimposed.
Mutsuo Nuriya, PhD, Masato Yasui, MD, PhD Department of Pharmacology School of Medicine, Keio University