Biological Microscopes

		Spectral type fluorescence detector	Filter type fluorescence detector
Laser light	Ultraviolet/Visible light laser	LD laser: 405nm: 50mW, 440nm: 25mW, 473nm: 15mW, 559nm: 15mW, 635mW, 20mW Multi Ar laser (457nm, 488nm, 515nm, Total 30mW), HeNe(G) laser (543nm, 1mW)
	AOTF laser combiner	Visible light laser platform with implemented AOTF system, Ultra-fast intensity modulation with individual laser lines, additional shutter control Continuously variable (0.1% - 100%, 0.1% increment), REX: Capable of laser intensity adjustment and laser wavelength selection for each region
	Fiber	Broadband type (400nm-650nm)
Scanning and Detection	Scanner module	Standard 3 laser ports, VIS - UV - IR Excitation dichromatic mirror turret, 6 position (High performance DMs and 20/80 half mirror), Dual galvanometer mirror scanner (X, Y) Motorized optical port for fluorescence illumination and optional module adaptation, Adaptation to microscope fluorescence condenser
	Detector module	Standard 3 confocal Channels (3 photomultiplier detectors) Additional optional output port light path available for optional units 6 position beamsplitter turrets with CH1 and CH2 CH1 and CH2 equipped with independent grating and slit for fast and flexible spectral detection Selectable wavelength bandwidth: 1-100nm Wavelength resolution: 2nm. Wavelength switching speed: 100nm/msec CH3 with 6 position barrier filter turret	Standard 3 confocal Channels (3 photomultiplier detectors) Additional optional output port light path available for optional units 6 position beamsplitter turrets with CH1 and CH2 CH1 to CH3 each with 6 position barrier filter turret (High performance filters.)
	Filters	High performance sputtered filters, dichromatic mirrors and barrier filters
	Scanning method	2 galvanometer scanning mirrors
	Scanning modes	Pixel size: 64 x 64 — 4096 x 4096 Scanning speed: 512 x 512 (1.1 sec., 1.6 sec., 2.7 sec., 3.3 sec., 3.9 sec., 5.9 sec., 11.3 sec., 27.4 sec., 54.0 sec.) 256 x 256 bidirectional scanning (0.064 sec., 0.129 sec.) 0.5 or 1 microsec dwell time per point for fast bidrectional scanning
		X,Y,T,Z,λ Line scanning: Straight line with free orientation, free line, Point scanning	X,Y,T,Z Line scanning: Straight line with free orientation, free line, Point scanning
	Photo detection method	2 detection modes: Analog integration and hybrid photon counting
	Pinhole	Single motorized pinhole pinhole diameter ø50 - 300μm (1μm step)	Single motorized pinhole pinhole diameter ø50 - 800μm (1μm step)
	Field Number (N.A.)	18
	Optical Zoom	1x - 50x in 0.1x increment
	Z-drive	Integrated motorized focus module of the microscope, minimum increment 0.01μm or 10 nm
	Transmitted light detector unit	Module with integrated external transmitted light photomultiplier detector and 100W Halogen lamp, motorized switching, fiber adaptation to microscope frame
Microscope	Motorized microscope	Inverted IX81, Upright BX61, Upright focusing nosepiece & fixed stage BX61WI
	Fluorescence illumination unit	External fluorescence light source with motorized shutter, fiber adaptation to optical port of scan unit Motorized switching between LSM light path and fluorescence illumination
System Control	PC	PC-AT compatible, OS: Windows XP Professional (English version), Memory: 2GB or larger, CPU:Pentium 3.2GHz or higher, Hard disk: 120GB or larger, Media: DVD Multi Drive, FV1000 Special I/F board (built-in PC), Graphic board: ATI RADEON X700 PRO
	Power Supply Unit	Galvo control boards, scanning mirrors and gratings, Real time controller	Galvo control boards, scanning mirrors
	Monitor	Monitor: SXGA 1280X1024, dual 19 inch or larger monitors
Optional unit	SIM Scanner	2 galvanometer scanning mirrors, pupil projection lens, built-in laser shutter, 1 laser port Fiber introduction of near UV diode laser or visible light laser, Optional: 2nd AOTF laser combiner
	TIRFM unit	Available laser: 405~633nm. Motorized penetration ratio adjustment. Automatic optical setting for TIRFM objectives.
	4th CH detector	Module with photomultiplier detector, barrier filter turret, beamsplitter turret mounted with 3rd CH light path
	Fiber port for fluorescence	Output port equipped with FC fiber connector (compatible fiber core 100 -125μm)

Software

Display	Dual monitor display
Optional Hardware	SIM scanner unit, 4ch detector. Fiber port
Image format	OIB/OIF image format 8/16 bit gray scale/index color, 24/32/48 bit color, JPEG/BMP/TIFF/AVI/MOV image functions Olympus multi-tif format
Image acquisition	Region designation: point, line, free line, clip, clip zoom Real-time image calculation: Kalman filtering, photon counting mode
	2-dimension: XY, XZ, XT and Xλ 3-dimension: XYZ, XYT, XYλ, XZT, XTλ and XZλ 4-dimension: XYZT, XZTλ and XYTλ
Image pixel format	64 x 64 — 4096 x 4096
Programmable Time Controller function	Time Controller function
Image display	Each image display: Single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/λ), LUT: individual color setting, pseudo-color, comment: graphic and text input
3D visualization and observation	Interactive volume rendering Free orientation of cross section display 3D animation, left/right stereo pairs, red/green stereoscopic images and cross section 3D and 2D sequential operation function
Spectral unmixing	2 Fluorescence spectral unmixing modes (normal and blind mode)
Image processing	Individual filter: average, low-pass, Sobel, Median, *Prewitt, 2D Laplacian, edge enhancement etc. Calculations: inter-image, mathematical and logical, DIC background leveling
Image analysis	Fluorescence intensity, area and perimeter measurement, time-lapse measurement
Statistical processing	2D data histogram display, colocalization
Others (options)	Off-line review station software

Objectives for BX and IX (using U-UCD8A, IX-LWUCDA and U-DICTS)

Description	N.A.	W.D.	Cover glass thickness	Immer-sion	Corre- ction ring	Condenser for BX U-UCD8A optical element	Condenser for IX IX-LWUCDA optical element	U-DICTS position
UPLSAPO 4x	0.16	13	—
UPLSAPO 10x2	0.40	3.1	0.17			U-DIC10	IX2-DIC10	normal
UPLAPO 10xO3	0.40	0.24	0.17	Oil		U-DP10	IX-DP10	normal
UPLAPO 10xW3	0.40	0.43	0.17	Water		U-DP10	IX-DP10	normal
UPLSAPO 20x	0.75	0.6	0.17			U-DIC20	IX2-DIC20	normal
UPLSAPO 20xO	0.85	0.17	—	Oil		U-DIC20	IX2-DIC20	normal
UPLSAPO 40x2	0.95	0.2	0.11-0.23		O	U-DIC40	IX2-DIC40	normal
UPLFLN 40xO	1.30	0.2	0.17	Oil		U-DIC40	IX2-DIC40	BFP1
APON 60xOTIRFM	1.49	0.08-0.14	0.13-0.19	Oil		U-DIC60	IX2-DIC60	BFP1
UPLSAPO 60xO	1.35	0.15	0.17	Oil		U-DIC60	IX2-DIC60	BFP1
UPLSAPO 60xW	1.20	0.28	0.15-0.2	Water	O	U-DIC60	IX2-DIC60	normal
UPLSAPO 100xO	1.40	0.12	0.17	Oil		U-DIC100	IX2-DIC100	normal

Low Auto-fluorescence Immersion Oil

See more

Immersion Oil	Refraction index (nd)	Abbe (νd)	Viscosity
IMMOIL-F30CC	1.5150±0.0008	41.0±1.2	450±100

Objectives for fixed stage upright microscopes (using WI-UCD, WI-DICTHRA)

Objective	N.A.	W.D.	DIC prism	Revolving nosepiece
MPLN5x	0.10	20.0	–	WI-SSNP, WI-SRE2
UMPlanFl10xW	0.30	3.30	U-LDPW10H	WI-SSNP, WI-SRE2
UMPlanFl20xW	0.50	3.30	U-LDPW20H	WI-SSNP, WI-SRE2
LUMPlanFl40xW	0.80	3.30	U-LDPW40H	WI-SSNP, WI-SRE2
LUMPlanFl60xW	0.90	2.00	U-LDPW60H	WI-SSNP, WI-SRE2
LUMPlanFl40xW/IR2	0.80	3.30	U-LDPW40H	WI-SSNP, WI-SRE2
LUMPlanFl60xW/IR2	0.90	2.00	U-LDPW60H	WI-SSNP, WI-SRE2
LUMPlanFl100xW	1.00	1.50	U-LDPW60H	WI-SSNP, WI-SRE2
XLUMPlanFl20xW	0.95*	2.00	U-LDPXLU20HR	WI-SNPXLU

* Note: These conditions are not met in confocal microscopy

Dimensions, weight and power consumption

	Dimensions(mm)	Weight (kg)	Power consumption
Microscope withScan unit	BX system: 320(W) x 580(D) x 565(H)	41	—
	IX system: 350(W) x 750(D) x 640(H)	51
Fluorescence illumination unit	Lamp: 180 (W) x 320 (D) x 235 (H)	6.7	100-120V/200-240V 260VA
	Power supply: 150(W) x 295(D) x 200(H)	4.6
Transmitted light detection unit	170(W) x 330(D) x 130(H)	5.9	—
Microscope control unit	125(W) x 330(D) x 215(H)	5.8	100-120/200-240V 350VA
FV Power Supply unit	180(W) x 328(D) x 424(H)	7.5	100-120/200-240V 400VA
FV Control unit (PC)	180(W) x 420(D) x 360(H)	11.2	100-120/200-240V 300VA
Monitor (dual)	415(W) x 216(D) x 435(H)	6.6	100-120/200-240V 100VA
Power supply unit for laser combiner	210 (W) x 300 (D) X 100 (H)	4.0	100-120V/200-240V 400VA
Laser combiner (with Laser heads)	514 (W) x 504 (D) x 236 (H)	45	—
Laser combiner (without Ar laser heads)	514 (W) x 504 (D) x 236 (H)	40	—
LD559 laser power supply	200 (W) x 330 (D) x 52 (H)	1.2	100-120V/200-240V 30VA
Multi Ar laser Power Supply	162(W) x 330(D) x 98(H)	3.2	100-120/200-240V 1500VA
HeNe G laser Power Supply	160(W) x 270(D) x 54(H)	1.4	100-120/200-240V 30VA
Compressor	307 (W) x 383 (D) x 399 (H)	18	100V 280VA

Recommended FV1000 system setup (IX81, BX61, BX61WI) (unit: mm)