Biological Microscopes

Suitable for multiple users

For the convenience of multiple researchers, each user may create an independent log in which contains individual settings such as the display window and toolbar. To change researchers, the new user simply logs in at software start-up.

Wide choice of scanning modes

Several scanning modes are available, including ROI, point and high-speed bi-directional scanning. These can be used together freely, in such combinations as XYZ, XYT, XYZT, XYλ, XYλT, etc.

Easy designation of scan area

The observation field of view and scanning area are displayed graphically, and settings can be altered while confirming the magnifications with the scroller. The operator can move the image scan area at will using the Pan button.
Rotation scanning of the image field is also possible.

Eliminate cross talk by using

Both frame and line sequential modes can be selected. The order of image acquisition, or image combinations, can be freely changed.

Free emission wavelength selection

When selecting fluorochromes from within the software, the fluorescence spectra are displayed and the ideal detection wavelengths are automatically selected. Naturally, manual adjustment of the wavelength emission range, in as little as 1nm step increments, is also possible in order to optimize acquisition of specific fluorescence emission peaks.

On-line help

The comprehensive On-line help explains each command's function and usage, as well as the overall flow of operations.

One-touch exchange between confocal and fluorescence observation

Since the FV1000 is fully automated, one-touch exchange between confocal and fluorescence observation is possible. In addition, microscope settings can be easily changed through the Microscope Controller.

Easy image acquisition for 3D, 4D and 5D series

Multi-dimensional image acquisition, such as λ series, Z series and timelapse, is easily performed.

Automatic contrast

Automatic contrast selects optimal photomultiplier voltage settings for each detection channel, simplifying image acquisition.

Real-time emission intensity graph display

For image acquisition in real time, live emission intensity is displayed in a graph. Since the images are captured using the full 12-bit capacity, this is also convenient for setting sensitivity levels.

2D View Analysis Tool

Background Correction : Subtracts background.		Series Analysis : Analysis of variation in intensity along Zaxis / time-axis in region designated as ROI.
Region Measurement : Measures size or intensity of region designated as ROI.		Line Series Analysis : Analysis of variation in intensity along Zaxis / time-axis on a designated line.
Intensity Profile : Displays intensity profile of region designated with ROI or Line.		Colocalization : Analysis of degree of overlap, between two channels, of pixels at or higher than a certain intensity.
Histogram : Displays histogram of intensity values of region designated as ROI.		Ratio : Create an image using the intensity ratio between two channels.

Easy image search

Explorer software provides simultaneous thumbnail image display, making it easy to search for previously stored data.

Data manager

The data manager provides thumbnail displays and different types of file information.

2D image display

The 2D control panel enables free manipulation of the image display. Tiling display or multi-dimensional image display can also be freely selected.

Re-use function

Previously-set scanning conditions can be recalled and applied to new or subsequent experiments.

File Input/Output

OIB (Olympus Image Binary format) is employed to acquire both scanning and microscope setting conditions together with image acquisition data. This software also handles all widely used image formats (TIF, BMP, JPEG, AVI, MOV etc) with high interchangeability.