Confocal Laser Scanning Biological Microscope FV1000 : Features (3)
- Multi-combiner
- SIM scanner
- Spectral and filter scan units
- Software
- Option Unit
Spectral and filter scan units provide two types of highprecision scanning.
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Variable Barrier Filter (VBF).
With the spectral detection system, once a fluorochrome combination is selected within the software, the ideal wavelength spectrum is automatically selected. Of course, manual adjustment of the wavelength range is also possible according to the fluorescence emission wavelength peaks. The spectral scan unit can be used to optimize image acquisition since the sensitivity of each channel can be adjusted with the same responsiveness as the filter version.
High performance mirror unit with special coating.
High-performance mirrors with a special new coating are introduced to all emission filters and dichromatic mirror units, achieving sharper transitions which were not possible with the conventional vacuum deposition method. In particular, these mirrors cover the full fluorescence emission spectrum for fluorescent proteins, enabling clear observation even with weak fluorescence emission and minimizing damage to live cells.
High sensitivity detection system.
High-sensitivity, high S/N ratio optical performance is achieved through integration of a pupil projection lens within the optics and employment of a highsensitivity photomultiplier and analog processing circuit with minimal noise.
Photon counting detection mode.
he Olympus original Hybrid Photon Counting Mode (HPCM) can successfully capture images even when fluorescence emission is weak. This mode optimizes photomultiplier control conditions to acquire images with minimum noise and high quantitative performance.
Original spectral system.
An independent photomultiplier is incorporated into the original optics for each channel. The special spectral scanning system uses a diffraction grating, which has excellent linear performance with no wavelength deviation and ensures high-precision, high-resolution, high-speed spectroscopy.
High-speed spectroscopy.
High-speed galvanometer diffraction grating delivers high wavelength change speed (100nm/ms), enabling very fast acquisition of XYλT images.
High-precision spectroscopy.
Cross talk derived from two fluorochromes with similar emission wavelength peaks can be clearly separated by the system's highly accurate 2nm wavelength resolution. Even when working with specimens whose fluorescence emission wavelength is similar to the excitation wavelength, it is possible to obtain images unaffected by the excitation light.
