Biological Microscopes

Rat kidney

Mouse cerebellum climbing fiber

Blue (DAPI): Nuclei
Green (Qdot 525): Aquaporin water channels 3 (aquaporin 3; AQP3)
Red (Qdot 655): Aquaporin water channels 2 (aquaporin 2; AQP2)
Data courtesy of: Dr. Kuniaki Takada
Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine

Visualized image of anterograde-labeled climbing fibers and their synapse terminal. Biotinylated-dextran amine (BDA) was injected into the inferior olivary nucleus of a mouse. After a few days for fixing, they were sliced thinly and dyed with TexasRed labeled avidin.
Data courtesy of:
Dr. Tetsuro Kashiwabara, Assistant Professor; and Dr. Akira Mizoguchi, Professor; Neuroregenerative medicine course, Mie University School of Medicine

Mouse cerebellum

Mouse embryo:
Posterior vena cava and somite

Triple stained image of mouse cerebellum using Calbindin (blue) FITC, VGluT1 (red) CY3, VGluT2 (green) CY5
Purkinje cell, parallel fiber terminal and climbing fiber terminal can be visualized.
Data courtesy of: Dr. Tasuke Miyazaki and Dr. Masahiko Watanabe
Department of Anatomy, Hokkaido University School of Medicine

AlexFluor488: ß-Catenin
AlexFluor 546: Vascular endothelial cell PECAM-1
TOTO-3: Nucleus
Data courtesy of: Dr. Masanori Hirashima
Developmental Biology, School of Medicine, Keio University

Osteoclast induced from rat monocyte in rat kidney

Cell division (HeLa cell)

Green (AlexaFluor488): beta3 integrin
Magenta (TexasRed): Osteopontin
Blue (DAPI): Nucleus
Data courtesy of: Dr. Keiko Suzuki,
Department of Pharmacology, Showa University School of Dentistry

Immunostaining of HeLa cell is done using mouse monoclonal antibody with splicing factor
SC35 and Alexa555 as secondary antibody. DNA is stained with DAPI. Characteristic patterns of intracellular localization are shown for interphase and mitotic period.
Green: DAPI (DNA) Red: Alexa555 (SC35, splicing factor)
Data courtesy of: Dr. Takeshi Fukuhara, Department of Functional Genomics,
Medical Research Institute & Laboratory of Gene Expression,
School of Biomedical Science, Tokyo Medical and Dental University Graduate School

Cerebellum Purkinje cell

Expression of specific Cre gene in neural stem cell of cerebellum Purkinje cell

Visualized overall images of Purkinje cell axon, cell body and dendrites.
Biotinylated-dextran amine was injected into mouse cerebellar nuclei. After a few days for fixing, they were sliced and anterograte labeled with TexasRed labeled avidin.
Data courtesy of:
Dr. Tetsuro Kashiwabara, Assistant Professor; and Dr. Akira Mizoguchi, Professor;
Neuroregenerative medicine course, Mie University School of Medicine

Pilidium larva of Micrura alaskensis

Cultured nerve cells derived from the mouse hippocampus

Pilidium larva of Micrura alaskensis (Phylum Nemertea) with developing juvenile inside
Courtesy of Dr. Svetlana Maslakova of the University of Washington and Dr. Mikhail V Matz of the Whitney Laboratory for Marine Bioscience, University of Florida.

Green (AlexaFluor488): Protein χ
Magenta (AlexaFluor568): MAP2