Biological Microscopes

World leading fluorescence performance - a vital key to modern life science research

The ideal in fluorescence observation is to capture high-contrast images with the lowest exposure to excitation light, thus minimizing the chances of cell damage and fluorescence fading. Olympus' UIS2 objectives are precisely designed to obtain bright images from weak fluorescence signals. This improvement in transmission is due to the increased S/N ratio of the UIS2 optics.

Improved performance of interference membrane-type fluorescence mirror units

S/N ratios are improved by applying new coating technology to the interference membrane of the mirror unit. S/N ratios are also increased by reducing the excitation (BP) and emission (BA) bandwidth by 6nm. The hard coat technique, applied to Olympus fluorescence mirror unit, prolongs the lifetime of the filter.

Highly-quality fluorescence mirror units for fluorescence proteins

The BX2 series mirror units are ideal for the wavelength characteristics of ECFP / EGFP / EYFP / DsRed.
The rapid rise time and high (90-95%) transmission factor efficiently transmit the fluorescence emitted from fluorescence proteins. This allows bright observation images even with weak excitation light, while preventing fluorescence fading and minimizing the chances of cell damage.

Stray light reducing function

The slight transmission of stray light when excitation light is reflected in the dichromatic mirror causes a rise in the level of noise. Olympus mirror units absorb more than 99% of this stray light through their light absorber.

The best S/N ratio...and the best fluorescence performance

UIS2 objectives' fluorescence S/N is improved by up to 1.5 times that of conventional objectives — by using strictly selected, low autofluorescence glass material, and adopting measures to minimize fluorescence from the reflection prevention coating and cementing material.
This has improved N.A. while also reducing autofluorescence, two benefits previously considered incompatible. As a result, even weak fluorescence emissions from weak excitation light are efficiently detected, enabling the UIS2 system to provide the best fluorescence imaging of live cells.

High N.A. objectives for fluorescence imaging

The BX2 series features the newly-developed PLAPON60XO objective, offering the world's highest N.A. (1.42) for fluorescence imaging, and the UPLSAPO100XO with high 1.4 N.A. and advanced universal features. In addition to their outstanding fluorescence S/N ratio, they enjoy UV transmission that was previously unachievable with 45mm parfocality. The UPLSAPO100XO objective is especially notable for maintaining its transmission factor up to the 340nm wavelength.

Low Auto-fluorescence Immersion Oil

The ability to reduce auto-fluorescence normally associated with immersion oil makes this product well suited for fluorescence microscopy.
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High sensitivity cooled CCD camera

The cooled CCD camera DP30BW is designed for fluorescence live cell imaging. It features high sensitivity, high-speed image acquisition and vibration-free operation.

Excellent trinocular tube performance even in the near infrared region

The trinocular tube U-TR30NIR improves the transmission factor and compensates for aberrations over a wider wavelength range. A new multi-coating is applied to the trinocular optical surfaces to widen the IR spectral characteristics and allow for observation of newly developed fluorochromes in the near-infrared region.

Camera adapter suitable for near infrared region

Users can choose from a variety of low magnification camera adapters with C-mount, all IR-compliant.
1.U-TV0.35XC-2 / 2.U-TV0.5XC-3 / 3.U-TV0.63XC / 4.U-TV1X-2+U-CMAD3

High transmission factor across a wide wavelength spectrum

The latest UIS2 objectives achieve a flat, high transmission factor over a wide wavelength spectrum, from visible to near infrared — thanks to the incorporation of a newly-developed ultra wide wavelength reflection prevention coating (UW multicoating).
The improvement in transmission factor on the near infrared region is especially notable, and typifies the high performance which makes UIS2 objectives the natural choice in many leading-edge research fields.

Up to near infrared compensation for chromatic aberration

UPLSAPO series objectives' Super Apochromat performance compensates for all chromatic aberrations, from visible to up to 1000nm wavelength light. Clear images without color shift are provided even in multi color observations that use fluorochromes whose excitation light wavelength is spread over a wide range. Imaging all the way from UV to IR can be performed with a single objective.