Fluorochrome data
| Fluorochrome | Excitation wavelength | Emission wavelength | Applicable excitation* | Comment |
|---|---|---|---|---|
| Fluorochrome antibody method | ||||
| Allophycocyanin(APC) | 650 | 660 | IY | Antibody labelling |
| 7-Amino-4-methylcoumarin- 3-acetic acid (AMCA) |
350 | 450 | U | Antibody labelling |
| BODIPY FL | 503 | 512 | B, IB | Antibody labelling |
| Cascade Blue | 376 | 425 | U | Antibody labelling |
| Fluorescein- isothiocyanate(FITC) |
490 | 520 | IB | Antibody labelling |
| Phycoerythrin B (PE-B) | 545 | 576 | G | Antibody labelling |
| Phycoerythrin R (PE-R) | 490,565 | 578 | G, IB | Antibody labelling |
| Rhodamine B-isothiocyanate (RITC) | 570 | 595 | G | Antibody labelling |
| Texas Red | 596 | 620 | IY | Antibody labelling |
| Tetramethylrhodamine- isothyocyanate (TRITC) |
541 | 527 | IG | Antibody labelling |
| Protein modification | ||||
| 8-Anilino-1-napthalene sulfonic acid Mg-salt (ANS) | 385 | 485 | V,U,BV | Hydrophobic part probe |
| Coumarin maleimide (CMP) | 385 | 465 | U | Thiol group |
| Cy 3 | 552 | 565 | IG | Amino group, antibody labelling |
| Cy 5 | 650 | 667 | IY | Amino group, antibody labelling |
| Dimethylaminonaphthalene- 5-sulfonic acid (DANS) |
380 | 520 | U | Thiol group |
| Dansyl chloride | 335 | 500 | U | Amine labelling |
| Dansyl hydrazine | 336 | 531 | U | Carbonyl group |
| Eosin-isothiocyanate | 524 | 548 | IB | Amine labelling |
| Fluorescamine | 390 | 460 | U, V | Amine labelling |
| Lucifer Yellow CH | 435 | 530 | BV, B | Aldehyde, ketone |
| Monochlorobimane | 380 | 461 | U | Thiol group |
| O-Phtalaldehyde (OPT) | 340 | 455 | U | Amino labelling |
| Resorufin | 570 | 585 | G | Thiol group |
| SITS | 350 | 420 | U | Amine labelling |
| Nuclei, nucleic acid | ||||
| Acridine Orange | 490 | 530, 640 | B,IB | Single/double-stranded nucleic acid |
| Acridine Yellow | 470 | 550 | B | Nucleic acid |
| Acriflavine-Feulgen | 430 | 515 | BV | DNA |
| Auramine O-Feulgen | 460 | 550 | B | DNA |
| 7-Amino Actinomycin D | 555 | 655 | IY,IG | G-C range |
| bis-Aminophenyl- oxabizole (BAO)-Feulgen |
380 | 470 | U | DNA |
| Chromomycin A3 | 450 | 570 | B, BV | G-C range |
| 4,6-diamidino-2- phenylindole HCI (DAPI) |
372 | 456 | U | A-T range |
| Ethidium Bromide | 545 | 605 | G, IG | DNA•RNA |
| Hoechst 33258 | 365 | 465 | U | AT range |
| Hoechst 33342 | 355 | 465 | U | AT range |
| Mithramycin | 395 | 570 | BV | G-C range |
| Olivomycin | 430 | 545 | BV | G-C range |
| Pararosaniline-Feulgen | 560 | 625 | G | DNA |
| Propidium Iodide | 530 | 615 | IB,G,IG | DNA•RNA |
| Pyronin Y | 540 | 570 | IG | DNA•RNA |
| Quinacrine Mustard | 385 | 525 | BV | AT range, Q band |
| Bone | ||||
| Acidic Fuchsin (Pararosaniline) | 540 | 630 | G | Calcification |
| Calcein | 495 | 520 | IB | Bone growth |
| Calcein Blue | 375 | 435 | U | Bone growth |
| Tetracycline | 390 | 560 | BV,U | Calcification |
| Xylenol Orange | 440,570 | 610 | G | Bone growth |
| Carbonydrate modification | ||||
| Acriflavine SO2,PAS | 430 | 515 | BV | Sugar |
| Cell viability | ||||
| Living cell staining | ||||
| Carboxyfluoresceindiacetate (CFDA) | 495 | 520 | IB | Esterase substrates |
| Fluoresceindiacetate (FDA) | 495 | 520 | IB | Esterase substrates |
| Calcein/AM | 495 | 520 | IB | Esterase substrates |
| BCECF/AM | 500 | 530 | IB | Esterase substrates |
| Acridine Orange | 490 | 590 | B,IB | Acid organelle |
| Dead cell staining | ||||
| Ethidium Bromide | 545 | 605 | G,IB | Cell membrane impermeability |
| Ethidium Homedimer | 528 | 617 | G,IB | Cell membrane impermeability |
| Propidium Iodide | 530 | 615 | G,IB | Cell membrane impermeability |
| Nerve cell tracers | ||||
| Evans Blue | 550 | 610 | G | Retrograde labelling |
| Fast Blue | 360 | 410 | U | Retrograde labelling |
| Granular Blue | 375 | 410 | U | Retrograde labelling |
| Lucifer Yellow CH | 430 | 535 | BV | Tracer |
| True Blue | 373 | 404 | U | Retrograde labelling |
| Membranes | ||||
| 8-Amino-naphthalene sulfonic acid (ANS) | 385 | 485 | V | Negative ionicity |
| DiI | 550 | 565 | G,IG | Positie ionicity,tracer |
| DiO | 484 | 501 | B,IB | Positie ionicity,tracer |
| Diphenylhexatrience (DPH) | 351 | 430 | U | Neutral |
| Filipin | 350 | U | Cholesterol | |
| Fluorescamine | 390 | 460 | U,V | Membrane surface protein |
| Merocyanine 540 | 500 | 572 | G | Membrane potential |
| Organelle | ||||
| Acridine Orange | 490 | 590 | B,IB | Lysosome |
| BODIPY FL | 503 | 512 | IB | Golgi apparatus |
| DiOC6 (3) | 480 | 501 | IB | Endoplasmic reticulum |
| Nile Red | 485-530 | 525-605 | B,G | Lysosome |
| Rhodamine 123 | 500 | 540 | IB | Mitochondria |
| Pyronin Y | 540 | 570 | IG | Mitochondria |
| Bacteria staining | ||||
| Acridine Orange | 490 | 530,640 | B,IB | Tubercle bacilli |
| Acridine Yellow | 470 | 550 | B | Tubercle bacilli, acid-fast bacteria |
| Acridine O | 460 | 550 | B | Tubercle bacilli, acid-fast bacteria |
| Berberine sulfate Hoechst 33258 | 430 | 550 | BV | General bacteria |
| Hoechst 33258 | 365 | 465 | U | Myoplasma detection |
| Others | ||||
| NBD-Phallacidin | 465 | 530 | B,IB | Actin fibers |
| Rhodamine-Phalloidin | 550 | 580 | G,IG | Actin fibers |
| Thioflavin T | 430 | 550 | BV | Amyloid |
| Ion concentrations | ||||
| Fluo 3 | 490 | 530 | IB/FLUO | Calcium |
| Fura-2 | 340/380 | 510 | U/FURA | Calcium |
| Indo-1 | 350 | 405/480 | B/BCECF | pH |
| BCECF | 430/480 | 530 | B/BCECF | pH |
| GFP (Green Fluorescent Protein) | ||||
| S65-T | 489 | 511 | IB | |
| EGFP | 488 | 507 | IB | |
